Fig. 1.
The T198I mutation does not impact on synaptophysin localisation. (A) Schematic of the structure of synaptophysin with the location of the T198I mutation indicated by an arrow. (B–D) Primary cultures of hippocampal neurons derived from synaptophysin knockout mice were transfected with either wild-type (WT) or T198I mutant synaptophysin-pHluorin (Syp-pH). Representative images of neurons transfected with either sypWT-pHluorin (B) or sypT198I-pHluorin (C) are displayed, which have been pseudo-coloured to indicate regions of high intensity in white. Scale bar equivalent to 10 μm. (D) Coefficient of variation (CV) analysis for either sypWT-pHluorin (Black) or sypT198I-pHluorin (red) is displayed ± SEM (WT n = 12, T198I n = 8; students t-test p = 0.92).