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. 2017 Dec;108:298–306. doi: 10.1016/j.nbd.2017.08.021

Fig. 5.

Fig. 5

The T198I mutation has no effect on SV exocytosis. Primary cultures of synaptophysin knockout hippocampal neurons were transfected with either WT (black) or T198I (red) syp-pHluorin (B) or co-transfected with sybII-pHluorin (A, B) or vGLUT1-pH (B) and wild-type mCerulean-tagged human synaptophysin (WT, black) or the T198I mutant (T198I, red). Neurons were challenged with a train of 300 action potentials (10 Hz) and after 3 min a pulse of a buffer containing 50 mM NH4Cl to unquench all pHluorin fluorescence was applied as indicated by bars. (A) Traces display the average fluorescent sybII-pHluorin response normalised to the total pool (ΔF/F0 (NH4) ± SEM). (B) The average peak height of sybII-pHluorin (sybII), vGLUT1-pHluorin (vGLUT1) or syp-pHluorin (syp) normalised to NH4Cl (ΔF/F0 (NH4) ± SEM) is displayed (sybII-pH; WT n = 9, T198I n = 14, vGLUT-pH; WT n = 9, T198I n = 8, syp-pH WT n = 12, T198I n = 8; all ns students t-test).