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. 2017 Dec;108:298–306. doi: 10.1016/j.nbd.2017.08.021

Fig. 7.

Fig. 7

Effect of multiple XLID synaptophysin mutations on sybII targeting and surface fraction. (A, B) Primary cultures of synaptophysin knockout hippocampal neurons were transfected with sybII-pHluorin and either wild-type mCerulean-tagged human synaptophysin (WT, black), truncation mutants (59*, purple and 136*, maroon) a point mutant (G217R, orange), a frame shift mutant (469 fs, green) or empty mCer vector (mCer, red). (A) Coefficient of variation (CV) analysis for sybII-pHluorin is displayed ± SEM (mCer n = 12, WT n = 11, 59* n = 13, 136* n = 13, G217R n = 10, 469 fs n = 12). *** = p < 0.001, * = p < 0.05 one-way ANOVA compared to WT, ### = p < 0.001, # = p < 0.05 one-way ANOVA compared to mCer. (B) Surface expression of sybII-pHluorin is displayed as a percentage of total ± SEM (mCer n = 12, WT n = 11, 59* n = 13, 136* n = 13, G217R n = 10, 469 fs n = 12, KO n = 12). *** = p < 0.001, * = p < 0.05 one-way ANOVA compared to WT, # = p < 0.05 one-way ANOVA compared to mCer.