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. Author manuscript; available in PMC: 2017 Nov 21.
Published in final edited form as: Chem Res Toxicol. 2016 Oct 25;29(11):1894–1900. doi: 10.1021/acs.chemrestox.6b00318

Table 2.

Kinetic Parameters for the dNTP Dependence of the Pol ν Catalyzed Insertion Opposite O2-Alkyl-dT

amplitude
burst rate constant
kss/[pol] s−1
Amax/[pol] KA(μM) kpol(s−1) KddNTP(μM)
dATP dG 0.49 ± 0.01 101 ± 6 32 ± 4 136 ± 58 0.020 ± 0.002
dCTP dG 0.65 ± 0.02 5.3 ± 1.1 59 ± 2 6.2 ± 1.1 0.038 ± 0.003
dGTP dG 0.55 ± 0.01 58 ± 7 28 ± 2 47 ± 15 0.020 ± 0.003
dTTP dG 0.57 ± 0.02 92 ± 12 25 ± 3 60 ± 28 0.017 ± 0.002
dATP O6-Me-dG 0.54 ± 0.02 106 ± 12 20 ± 3 93 ± 54 0.011 ± 0.003
dCTP O6-Me-dG 0.62 ± 0.02 5.8 ± 0.9 39 ± 4 30 ± 11 0.041 ± 0.01
dGTP O6-Me-dG 0.58 ± 0.02 63 ± 9 19 ± 1 40 ± 16 0.021 ± 0.002
dTTP O6-Me-dG 0.62 ± 0.0 38 ± 5 14 ± 2 140 ± 50 0.011 ± 0.003
dATP O6-POB-dG 0.34 ± 0.02 88 ± 22 0.0014 ± 0.0001 38 ± 7 nd
dCTP O6-POB-dG 0.73 ± 0.04 47 ± 11 0.0015 ± 0.0001 146 ± 42 nd
dGTP O6-POB-dG 0.37 ± 0.01 240 ± 31 0.0013 ± 0.0002 nd
dTTP O6-POB-dG 0.63 ± 0.03 120 ± 18 0.00085 ± 0.00005 nd
dATP dT 0.74 ± 0.04 5.1 ± 1.8 62 ± 5 26 ± 7 0.027 ± 0.004
dCTP dT 0.51 ± 0.02 42 ± 9 13 ± 1 100 ± 21 0.008 ± 0.002
dGTP dT 0.69 ± 0.01 17 ± 2 10 ± 1 101 ± 41 0.007 ± 0.003
dTTP dT 0.54 ± 0.02 27 ± 6 15 ± 1 118 ± 27 0.012 ± 0.002
dATP O2-Me-dT 0.55 ± 0.02 39 ± 7 < 50 0.00150 ± 0.00014 95 ± 32 nd
dCTP O2-Me-dT 0.35 ± 0.01 16 ± 5 < 50 0.00113 ± 0.00002 84 ± 7 nd
dGTP O2-Me-dT 0.83 ± 0.02 24 ± 3 < 50 0.00212 ± 0.00016 78 ± 22 nd
dTTP O2-Me-dT 0.72 ± 0.02 22 ± 4 < 50 0.00238 ± 0.00009 88 ± 12 nd
dATP O2-POB-dT 0.37 ± 0.05 78 ± 9 0.00150 ± 0.00014 50 ± 22 nd
dCTP O2-POB-dT 0.16 ± 0.01 42 ± 11 0.00117 ± 0.00002 45 ± 4 nd
dGTP O2-POB-dT 0.44 ± 0.02 19 ± 4 0.00122 ± 0.00006 13 ± 6 nd
dTTP O2-POB-dT 0.21 ± 0.01 46 ± 9 0.00115 ± 0.00002 72 ± 4 nd