Fig 6. P. aeruginosa LESB58 was present two days post-infection when delivered with alginate.
Mice were given alginate or alginate mixed with P. aeruginosa LESB58 (7 x 106 CFU/mouse) IN at 0 h, then euthanized and samples processed at 42 h. (A) CFU counts from the lung homogenate. (B) Health scores over the course of the experiment. (C) Percentage of original weight for mice over the course of the experiment. ELISAs were performed for MCP-1 in lung homogenate (D) and serum (E); KC in lung homogenate (F) and serum (G); IL-6 in lung homogenate (H) and serum (I); and TNF-α in lung homogenate (J). Data represent mean ± SEM for n = 4 or 16 mice per condition from the combination of two experiments and were analyzed using unpaired t-test with Welch’s correction for 6A and 6D-6J, and with a repeated measures two-way ANOVA with Sidak’s multiple comparison for 6B and 6C. **: p ≤ 0.01, ***: p ≤ 0.001, ****: p ≤ 0.0001.