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. 2017 Nov 6;12(11):e0187544. doi: 10.1371/journal.pone.0187544

Fig 4. Mass spectrometric analysis of products generated from (GlcNAc)5.

Fig 4

Reaction products generated upon treating (GlcNAc)5 with ArCE4A were labeled with AMAC and analyzed by MS. (a) MS1 spectra of AMAC labeled reaction products at different reaction time points, showing appearance of mono- and di-deacetylated products. (b) Representative MS2 spectrum for the A4D1 peak at m/z 1186 from MS1 spectra (1h reaction time). (c) Representative MS2 spectrum for the A3D2 peak at m/z 1144 from MS1 spectra (24h reaction time). Bold signals in (b) and (c) correspond to the mass of the indicated CHOS plus AMAC and hydrogen. Reaction mixtures contained 2 mM A5 and 300 nM ArCE4A and were incubated at 37°C.