(A) The structural alignment of IBPv_a, IBPv_b, ColAFP, LeIBP, FfIBP, TisAFP6 and TisAFP8. Residues were colored by Qres, which was related to the distance of Cα after the alignment. Blue-white-red indicated from less deviance to large deviance between the Cα of aligned residues. (B) IBPv_a, IBPv_b, ColAFP, LeIBP, and TisAFP6 were aligned by their structure data, but were colored according to primary sequence similarity using BLOSUM 60. Blue-white-red indicated from high similarity to low similarity. (C) The structural alignment of the hyperactive β helical AFPs. ColAFP, FfIBP, TisAFP8 and IBPv_b. Residues were colored by Qres values. Blue-white-red indicated from less deviance to large deviance between the Cα of aligned residues.