Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 6;12(11):e0186924. doi: 10.1371/journal.pone.0186924

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Alam et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 1 — pCMV6-FLAG-OATP1B1 (A) or pCMV6-FLAG-OATP1B3 (B) vectors were transfected into HEK293 cells, either alone or in combination with HA-Ub expression vector. Transfection with empty vector pCMV6 served as the vehicle control in all experiments. Whole cell lysates (WCL) were subjected to immunoprecipitation (IP) with HA or FLAG antibody, followed by immunoblotting (IB) with FLAG or HA antibody, respectively. After stripping, the same blot was re-probed with HA or FLAG antibody, as indicated in the figure. Immunoblots of HA and FLAG were also conducted using WCL with β-actin serving as the loading control. Representative immunoblot images from n = 3 independent experiments are shown.