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. Author manuscript; available in PMC: 2017 Nov 6.
Published in final edited form as: Free Radic Biol Med. 2016 Aug 20;99:296–307. doi: 10.1016/j.freeradbiomed.2016.08.022

Fig. 8. The GSH-precursor NAC rescues post-radiation survival but the GSH inhibitor-BSO reduces survival of Cebpd-KO MEFs.

Fig. 8

WT and KO MEFs were treated with NAC (5 mM) for 2 h prior to IR exposure (10 Gy) and analyzed at indicated timepoints for (A) apoptosis using FITC Annexin V; (B) WT and KO MEFs were treated with NAC (5 mM) for 2 h prior to irradiation at 2 Gy and re-seeded for clonogenic assay 3 h post-irradiation and (C) cells were also harvested for GSH measurements; (D) KO and WT MEFs were treated with BSO (10 µM) for 24 h prior to irradiation at 2 Gy and re-seeded for clonogenic assay 3 h post-irradiation. The data for A and C are plotted as an average of 2–3 dishes ± S.E.M. The data for B and D are plotted as an average of 6 dishes each for the 2 biological replicates ± S.E.M.