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. 2017 Nov 2;101(5):716–724. doi: 10.1016/j.ajhg.2017.09.014

Figure 3.

Figure 3

Recombinant Protein Variants of DHX30 Initiate the Formation of SG-like Cytoplasmic Aggregates

(A) Immunocytochemical detection of DHX30-GFP fusion proteins (GFP, green), mitochondria (Mito, red), and endogenous ATXN2 (blue) in transfected U2OS cells. Regions shown at high magnification in the rightmost panels are indicated by boxes. Whereas wild-type DHX30-GFP preferentially resides throughout the cytoplasm and GFP accumulates in nuclei, recombinant protein variants of DHX30 induce the genesis of cytoplasmic foci containing endogenous SG-marker ATXN2 (arrowheads). Nuclei are identified via DAPI staining (gray).

(B) Bar graph indicating the percentage of transfected cells, in which recombinant GFP proteins induce the emergence of SG-like structures. Vertical bars indicate SD. Statistical analysis was performed using unpaired Student’s t test to individually compare each protein variant to wild-type DHX30-GFP (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; n > 300 from two independent transfections).