Figure 4.

SG Formation Initiated by Protein Variants of DHX30 Selectively Inhibits Global Translation in Transfected U2OS Cells

Puromycin incorporation assay in U2OS cells expressing DHX30-GFP fusion proteins (GFP, green). Translation is monitored by staining against puromycin (Puro, red), SGs are detected by ATXN2 (blue) and nuclei via DAPI staining (gray). While cells expressing wild-type DHX30-GFP or GFP display puromycin labeling comparable to neighboring untransfected cells, puromycin incorporation is strongly diminished in cells expressing recombinant protein variants of DHX30. Arrows indicate transfected cells. Note the correlation between SG assembly and lack of puromycin staining.