Skip to main content
. 2017 Nov 2;8:1462. doi: 10.3389/fimmu.2017.01462

Figure 1.

Figure 1

DC-mediated Nlrp10 expression triggers IFNγ production by antigen-specific T cells. (A–C) IFNγ released by OT-II T cells co-cultured with immature (A,B) or mature (C) WT or Nlrp10−/− DCs loaded with increasing OVA concentrations (1.62–25 µg/ml) (A) and at different DC:OT-II T-cell ratios (B,C) measured in culture supernatants after 3 days by ELISA. Data presented are from single experiments and are representative of three independent experiments. (D) Expression of T-cell activation markers 3 days after incubation with OVA-loaded WT or Nlrp10−/− DCs. (E) IFNγ production by OT-II T cells co-cultured for 3 days with different amounts of splenic DCs isolated from WT or Nlrp10−/− mice and loaded overnight with OVA antigen (25 µg/ml). Data are expressed as the means ± SD. *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations: DC, dendritic cells; WT, wild-type; MFI, median fluorescence intensity; OVA, ovalbumin.