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. 2017 Nov 2;8:1462. doi: 10.3389/fimmu.2017.01462

Figure 5.

Figure 5

Nlrp10−/− T cells show impaired IL-12 production and IFNγ release in vivo in response to TLR9 stimulation. (A) IL-12p40, IL-12p70 and IL-6 levels were measured in the sera of WT and Nlrp10−/− mice treated with CpG DNA for 3 h. Data are expressed as the mean ± SD. (B) Expression of Il12a and Il12b in splenic DCs isolated from WT and Nlrp10−/− mice 3 h after CpG DNA treatment. (C,D) Schematic of the adoptive transfer protocol. WT and Nlrp10−/− mice were adoptively transferred with CD4+ OT-II T cells and then exposed 24 h later to OVA plus CpG DNA via intraperitoneal injection. IFNγ production by OT-II T cells was evaluated by ELISA after in vitro re-stimulation for 3 days with WT DCs loaded or not with OVA. *p < 0.05; ***p < 0.001. Abbreviations: DCs, dendritic cells; OVA, ovalbumin; WT, wild-type.