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. 2017 Nov 2;8:1462. doi: 10.3389/fimmu.2017.01462

Figure 6.

Figure 6

Nlrp10−/− DCs have attenuated capacity to stimulate a Mtb-specific CD4+ T-cell response. (A) IL-12p70, IL-6, IP10 and MCP1 levels in lung homogenates of WT and Nlrp10−/− mice at different times after Mtb infection, as determined by ELISA. (B) Il12b and Ifng mRNA expressions in lung leukocytes isolated from Mtb-infected WT and Nlrp10−/− mice, as determined by semi-quantitative RT-PCR. (C) Il12a and Il12b mRNA expressions in bone marrow-derived dendritic cells (BMDCs) infected with Mtb, as assessed by semi-quantitative RT-PCR. (D) CFUs measured in Nlrp10−/− and WT BM-derived DCs 24 and 48 h after Mtb infection. (E) Lung leukocytes collected from Nlrp10−/− and WT mice 35 days post-Mtb infection were re-stimulated in vitro with Mtb-infected WT or Nlrp10−/− DCs for 16 h. The percentage of IFNγ-producing CD4+CD137+ T cells was assessed by flow cytometry. Data represent the mean ± SE. *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations: DCs, dendritic cells; FACS, florescence-activated cell sorting; Mtb, Mycobacterium tuberculosis; WT, wild-type; CFUs, colony-forming units.