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. 2017 Nov 2;8:2131. doi: 10.3389/fmicb.2017.02131

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Copyright © 2017 Cabello-Yeves, Ghai, Mehrshad, Picazo, Camacho and Rodriguez-Valera.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(Left), relative abundance (%) of total Verrucomicrobia (Eub338III) and specific Verrucomicrobia-Tous-C9LFEB (VrmC9to, 4.63 Mb of estimated genome size) and Opitutae-AMD-G3 (VrmG3Ama, 2.13 Mb of estimated genome size) relative to total heterotrophic picoplankton (HPP) for (A) Tous and (B) Amadorio reservoirs. (Right), microscopic fluorescence in situ hybridization images of samples from (A) Tous reservoir and (B) Amadorio reservoir. The micrographs show two pairs of identical microscopic fields, with samples stained with DAPI (up) and with the specific designed probes labeled with Cy3 (down). White arrows mark VrmC9To (A) and VrnG3Ama (B). Bar: 5 μm (all four pictures).