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. 2017 Nov 2;8:882. doi: 10.3389/fphys.2017.00882

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Copyright © 2017 Khalyfa, Poroyko, Qiao, Gileles-Hillel, Khalyfa, Akbarpour, Almendros, Farré and Gozal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of IN exposures on vWAT macrophage and Treg populations using flow cytometry. Panel (A) is a representative example for CD11C⁺ and CD206⁺ that were used for double positive for macrophages. M1 and M2 macrophages were also identified as CD11c⁺ or CD206+ cells, respectively. Panel (B) is a representative example for resident macrophages (CD64). Panel (C) is a representative example for M1-like metabolic macrophages (CD36hi ++) in vWAT. Blue color is for SC and red color for IN. Panel (D) is a representative example for Tregs (FoxP3 + and CD4+ cells) in vWAT. Data are shown as means ± SD (n = 8/experimental group). ^*Indicates statistical significance; p < 0.01.