Figure 2.

miR-34a and miR-34c-5p downregulate the expression of ATG4B

(A, C) qPCR analysis for ATG4B mRNA levels in HeLa and SKOV3 cells after transfection with equal amount of indicated plasmids and/or RNA oligos for 24 hr. ATG4B mRNA level was normalized with β-actin mRNA, and the data were represented as fold induction over the NC control

(B, D)Western blot analysis for ATG4B protein level in HeLa and SKOV3 cells after transfection with equal amount of indicated plasmids and/or RNA oligos for 24hr