Figure 3.

Rapamycin-induced autophagy is accompanied with the upregulation of ATG4B and the downregulation of miR-34a/miR-34c-5p

(A) HeLa and SKOV3 cells were treated with 0.5μMrapamycin for 6h or 12hr. Total protein was extracted and Western blot was used to detect the protein level of LC3 62 and ATG4B, taking tubulin as a loading control

(B, C) qPCR analysis for ATG4B mRNA and miR-34a/miR-34c-5p expression level. The relative level was determined with the 2^-ΔΔCT method, and the data were represented as fold induction over the NC control