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. 2017 Nov 2;8:1458. doi: 10.3389/fimmu.2017.01458

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Copyright © 2017 Pereiro, Romero, Díaz-Rosales, Estepa, Figueras and Novoa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Modulation of Nkl during vaccination and/or infection in different tissues by flow cytometry and qPCR. (A–C) head kidney; (D–F), spleen; (G–I), blood. (A,D,G) represent the percentage of Nkl-positive cells in the three tissues. (B,E,H) represent the median value of the intensity of fluorescence in positive cells in the three tissues. (C,F,I) represent the expression of nkl in the tissues by qPCR. The positive region in the FL1-H histogram was adjusted using the signal registered in the same samples stained with the preimmune serum. Gating values for the positive region were 300-9910 for the head kidney, 514-9910 for spleen, and 262-9910 for blood samples. In all figures, the mean value of six samples plus SD is represented. Asterisks indicate significant differences (p < 0.05) between experimental groups. Nkl, Nk-lysin; qPCR, quantitative polymerase chain reaction.