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. 2017 Nov 6;8:1333. doi: 10.1038/s41467-017-01080-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Increased XPC expression upon ASH1L depletion. a Immunoblot analysis of XPC, XPD and DDB2 in whole cell lysates. HeLa cells were pre-treated with siRNA targeting ASH1L or non-coding control RNA (NC), UV-irradiated (10 J m^–2) 48 h later and collected at different times. GAPDH and H3 were used as loading controls. b Level of XPC and DDB2 normalized to H3 (n = 4 independent experiments). Quantifications include both unmodified and ubiquitinated XPC. Protein levels in unirradiated controls are set to 100% and error bars show s.e.m. (one-sample t-test)