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. 2017 Oct 4;6(11):2635–2645. doi: 10.1002/cam4.1174

Figure 2.

Figure 2

Effects of cisplatin (CDDP) on RasR cells. (A) Relative survival of RasR cells after exposure to the indicated concentrations of CDDP for 24 h. (B) Relative sphere growth after 7 days of drug treatment. Representative images of spheres are also shown. Scale bars, 300 μm. (C) Flow cytometric analysis of cell cycle profile for RasR cells exposed to 0 or 10 μmol/L CDDP for 48 h. (D) Overlay of red fluorescence and phase‐contrast images (as well as fluorescence images alone [insets]) for explants treated with the indicated concentrations of CDDP for 0 or 4 days. Scale bars, 300 μm. The change in tumor area between day 0 and day 4 is also shown in a box‐and‐whisker plot and with individual values represented by colored circles. *P < 0.05. (E) Immunohistochemical staining of cleaved caspase 3 in tumor‐bearing explants from one experiment presented in (D), after fixation on day 4. Staining of the control left hemisphere is also shown. Scale bars, 100 μm. (F) Immunohistochemical staining for cleaved caspase 3 around the lateral ventricle (LV) walls and blood vessels of tumor‐free explants treated with vehicle, 20 μmol/L staurosporine (STS, positive control), or 50 μmol/L CDDP. Scale bars, 20 μm. (G) Immunohistofluorescence staining for γH2AX and (H) quantification of the proportion of γH2AX‐positive tumor cells after immunohistochemical staining of explants from (D). Scale bars, 100 μm. *P < 0.05.