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. 2017 Nov 6;7:14586. doi: 10.1038/s41598-017-14698-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of the digital microfluidic (DMF) system for loop-mediated isothermal amplification (LAMP) reaction and melting curve analysis (MCA) using Molecular Beacon (MB) DNA probes. (a) 3D schematic of the DMF chip. (b) Side view of the DMF chip and the control electronics of droplet actuation and heating. (c) Detailed illustration of the reaction chamber of the DMF chip. (d) Schematic of true positive (TP) and false positive (FP) LAMP products with and without MB binding. (e) Data example obtained from on-chip LAMP amplification and MCA for TP and FP samples.