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. 2017 Nov 6;7:14586. doi: 10.1038/s41598-017-14698-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LAMP primers, Molecular Beacon (MB) DNA probes design, and off-chip LAMP reactions. (a) Schematic of Molecular Beacon DNA probes and LAMP products (partial) hybridization. (b) Part of the nucleic acid sequence of T. brucei chromosome 11 and loci of LAMP primers and probes. (c) Amplification curves of off-chip optimized LAMP reactions (SYBR Green I fluorescence) with no MB probe, LF probe, or LB probe. LAMP reactions were run in duplicate. (d) Off-chip melting curves of LF probe and LB probe after implementing LAMP reactions. Inset shows the melting peaks of LF probe and LB probe.