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. 2017 Nov 6;7:14517. doi: 10.1038/s41598-017-15160-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The kinetic expression of potential miR-10a target genes in miR-10a transient knockdown or miR-10a overexpressing human mesangial cells. Human mesangial cells (HMCs) were transfected with miR-10a inhibitor (▲), miR-10a mimic (▼) or scramble negative controls (●) using Lipofectamine RNAiMAX (siRNA concentration = 5 pmol). The total RNA including miRNA was collected at 24, 27, 30, 36 and 48 hours after transfection. Fold changes were compared between samples at the same time points using the unpair t-test. Data are shown as mean ± S.E.M. of a minimum of three biological replicates from three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001 versus scramble controls.