Table 2.

Primers used for RT-PCR and in PCR gene amplification for sequencing.

No	Gene	Name	Primer sequence
Primers for RT-PCR
1	OPT1	OPT1RTPCRFor	5′-GTATGATGTTGTTCAAGACCTTCGGATAC-3′
2	OPT2	OPT1RTPCRRev	5′-GCAGCAAACTGCGCCCAAAAC-3′
3	OPT3	OPT2RTPCRFor	5′-CCAAAACCTTGCAGTAGTCACTGG-3′
		OPT2RTPCRRev	5′-GTTAACTGGATTGAAAAATGGTACCTG-3′
		OPT3RTPCRFor	5′-GGACAGGATATTTACCAATCAACGATAATGG-3′
		OPT3RTPCRRev	5′-AACCAAGTTGGCAGCGGTATAG-3′
4	PTR2	PTR2RTPCRFor	5′-ATGAGCAATCACCATGTGATAC-3′
5	PTR22	PTR2RTPCRRev	5′-ATGCAGCGGAGAAAGCAGAC-3′
		PTR22RTPCRFor	5′-CTTACTTGCTTACGTTCTGCTTCTTC-3′
		PTR22RTPCRRev	5′-CCGTTTATGATACCGACCCAAACAC-3′
Primers for PCR gene amplification
1	OPT1-N-terminus	OPT1S1For	5′-CACTATTGATTCCATTCCTAACATT-3′
		OPT1S1Rev	5′-CAACGATGCGATAAGGTCAC-3′
	OPT1-C-terminus	OPT1S2For	5′-ATCCACCACCTTTGCC-3′
2	OPT2-N-terminus	OPT1S2Rev	5′-GCAATAATGTGTGTTTGTGTG-3′
3	OPT2-C-terminus	OPT2S1For	5′-ATGGTTTTAAAAGATTGCAAATTG-3′
	OPT3-N-terminus	OPT2S1Rev	5′-GTTAACTGGATTGAAAAATGGTACCTG-3′
	OPT3-C-terminus	OPT2S2For	5′-CCAAAACCTTGCAGTAGTCACTGG-3′
		OPT2S2Rev	5′-CTAAGGGAAATGACCGATCCTTGG-3′
		OPT3S1For	5′-ATGGATGAAAAAAATCCAACAACAGAATTAAGTG-3′
		OPT3S1Rev	5′-AACCAAGTTGGCAGCGGTATAG-3′
		OPT3S2For	5′-GGACAGGATATTTACCAATCAACGATAATGG-3′
		OPT3S2Rev	5′-CTAAGGGAAGTGACCAACTCTTGG-3′
4	PTR2	PTR2SeqFor	5′-GTTCTTATTGTTACTTCAGCCTC-3′
5	PTR22	PTR2SeqRev	5′-GAGATAAGATGTATGTTTGCAAATGTC-3′
		PTR22SeqFor	5′-ATGTCCACAGAAGAGAAACATCTGCA-3′
		PTR22SeqRev	5′-CTATGCATGGATTTGCGTGACGGAG-3′