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. 2017 Nov 6;7:14540. doi: 10.1038/s41598-017-14860-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Retinal ganglion cells count (for FluoroGold-positive cells) in experimental groups. Control – healthy; G14/G21/G28/G35 – glaucoma with PBS injection, euthanasia on 14^th, 21^st, 28^th or 35^th day respectively; PNE 2D/14 – glaucoma with PNE injection on the 2^nd, euthanasia on the 14^th day; PNE 7D/21 - glaucoma with PNE injection on the 7^th, euthanasia on the 21^st day; PNE 7D/28 - glaucoma with PNE injection on the 7^th, euthanasia on the 28^th day; PNE 7D/35 - glaucoma with PNE injection on the 7^th, euthanasia on the 35^th day; PNE 14D/28 - glaucoma with PNE injection on the 14^th, euthanasia on the 28^th day; PNE 14D/35 - glaucoma with PNE injection on the 14^th, euthanasia on the 35^th day. PNE – Peripheral Nerve Extract. Retinal cross-sections: in upper panel represent FluoroGold labeled RGC in GCL of healthy and glaucomatous retinas; in lower panel represent FluoroGold and DAPI labeled RGC in GCL of healthy and glaucomatous retinas. GCL–Ganglion Cells Layer; RGC–Retinal Ganglion Cells.