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. 2017 Nov 6;7:14540. doi: 10.1038/s41598-017-14860-5

Figure 6.

Figure 6

Ex vivo retinal explants cultured for 7 days in standard Neurobasal A medium with no treatment (A), treated with 1 µg/ml of Metallothionein II (B) and treated with 1 µg/ml of MT II and 10 µl/10 ml Gentamycin (C). Immunofluorescent staining for β3tubulin with AlexaFluor 594. Scale bar = 20 μm. NA – explants in standard Neurobasal A medium with no treatment; NAMT – explants treated with 1 µg/ml of Metallothionein II; NAMTG – explants treated with 1 µg/ml of MT II and 10 µl/10 ml Gentamycin; MT – metallothionein 2; G – Gentamycin. Statistical analysis indicated with: double asterisk (**) – p < 0.03, triple asterisk (***)–p < 0.005, independent t-test.