Skip to main content
. 2017 Nov 6;7:14571. doi: 10.1038/s41598-017-14901-z

Figure 2.

Figure 2

MC and mitochondrial activity. (A) – Representative image of oxygen consumption rate (OCR) assessment in wt HCT116 cells after treatment with colcemid (0.1 µg/ml, 0.01 µg/ml) for 24 h; (B) - Quantification of OCR data; (C) – Mitochondrial membrane potential (MMP) in wt HCT116 and HCT116 σ−/− cells treated with colcemid (0.1 µg/ml) for 24 h; (D,E) – ROS level in wt HCT116 and HCT116 σ−/− cells after treatment with doxorubicin (600 nM) or colcemid (0.1 µg/ml) for 24 h. In (D) – ROS level was measured after transfection with pHyPer-dMito plasmid, in (E) – using MitosoxRed; (F) – representative data of mitochondrial structure remodeling after treatment with doxorubicin (600 nM) and colcemid (0.1 µg/ml) for 24 h. Mitochondria were stained by MitoTracker® Red CMXRos. Magnified area outlined by white dotted rectangle. In (B,E) – data presented as mean ± SEM, n = 3 for each cell type; *p < 0.05 (U test).