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. 2017 Nov 6;7:14571. doi: 10.1038/s41598-017-14901-z

Figure 4.

Figure 4

Modulation of MC by Bcl-xL and Mcl-1 overexpression. (A) – Representative images of cells transfected with pEYFP-C1-Dest-hMcl-1 or pEGFP-Bcl-xL-N1 plasmids. (B) – Apoptotic cell death, assessed by the analysis of sub-G1 population in cells treated with doxorubicin (600 nM) or colcemid l (0.1 µg/ml) 24 h after colcemid and 48 h after doxorubicin treatments. (C) – The development of MC in transfected wt HCT116 and HCT116 σ−/−cells treated with doxorubicin and colcemid for 24 h. In B, data presented as mean ± SEM, n = 5 for each cell type; *p < 0.05 (U test); in C, mean ± SEM, n = 12 for each cell type; *p < 0.01 (U test).