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. 2017 Nov 6;7:14584. doi: 10.1038/s41598-017-14607-2

Figure 1.

Figure 1

Inhibition of AR impairs the growth of HER2 breast cancer cells. (a,b) Knockdown of AR with shRNAs reduced the growth of HCC1954 and SKBR3. HCC1954 (a) and SKBR3 (b) cells were infected with lentivirues expressing control shRNA (Con-sh) and two different AR shRNAs (sh1 and sh2) and immunoblotted with anti-AR and anti-tubulin antibodies (a,b bottom panels). All cropped blots were run under the same experimental conditions. The original blots are included in Supplementary Figure S2. Colony formation assay was used to assess the growth of HCC1954 and SKBR3 cells expressing Con-sh, sh1, and sh2 (a,b top panels). AR antagonist, enzalutamide (Enza), together with trastuzumab (Tras) inhibited the growth of HCC1954 (c) and SKBR3 (d) cells. Cells were subjected to colony formation assay in the presence of Vehicle, 20 μM Enza, 20 μg/ml Tras, and 20 μM Enza + 20 μg/ml Tras for 6 days before analyzed with crystal violet staining. Data shown is the representative from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, one-way ANOVA.