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. 2017 Nov 6;7:14584. doi: 10.1038/s41598-017-14607-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of AR expression inhibits HER2 phosphorylation and signaling without affecting HER3 protein expression in HCC1954 cells. Equal amounts of lysates from HCC1954 cells expressing con-sh, AR sh1, and AR sh2 were immunoblotted with the indicated antibodies (a). All cropped blots were run under the same experimental conditions. The original blots are included in Supplementary Figure S5. The relative levels of AR (b), P-HER2 (c), P-Akt (d), and P-Erk (e) were quantified by normalizing with the Tubulin levels. Data shown is the average from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, one-way ANOVA.