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. 2017 Nov 6;7:14543. doi: 10.1038/s41598-017-15149-3

Figure 3.

Figure 3

Selecting a higher apidaecin-production mutant with lower integration plasmid loss. (A) NTG mutagenesis was used to select a higher apidaecin-production mutant strain. (B) Antimicrobial activity of AP26, C and APmu4 fermentation supernatants, sampled 72 h after induction and diluted 10-fold in the water, against E.coli. (C) The percentage of integration plasmid loss rates of AP26 and APmu4 were calculated at 60 and 72 h respectively. (D) Tricine-SDS–PAGE analysis of apidaecin in fermentation supernatants. Lane M: 10 μl of protein molecular weight marker. Lane C: 10 μl of P. pastoris C strain 72 h fermentation supernatants. Lane 24h-96h: 10ul of APmu4 fermentation supernatants taken at 24 h, 48 h, 72 h and 96 h.