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. 2017 Nov 6;7:14543. doi: 10.1038/s41598-017-15149-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Purification and identification of apidaecin. (A) The samples from each step during the purification processes were monitored by Tricine-SDS–PAGE. (B) Protein fractions with antimicrobial activity against E. coli eluted from an SP FF column were further purified by HPLC at a flow speed of 1 ml/min. Buffer A for the HPLC was 0.1% trifluoroacetic in 100% water. Buffer B for the HPLC was 0.1% trifluoroacetic in 100% acetonitrile. (C) Apidaecin was detected by ESI-MS from the HPLC fraction denoted with an asterisk.