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. 2017 Nov 6;7:14579. doi: 10.1038/s41598-017-15074-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NMI enhances TNF-α plus CHX induced HeLa cell apoptosis. (A) The NMI-overexpressing HeLa cells (HeLa-NMI) showed an increased apoptotic morphology after TNF-α plus CHX treatment. HeLa-Ctrl and HeLa-NMI cells were plated in 12-well plates. The following day, the cells were treated as indicated (TNF-α: 5 ng/ml, CHX: 5 μg/ml) for 12 h or 24 h, and the cells images were then taken with a Nikon-TE2000 microscope. (B) The cell death in (A) was quantified by a Trypan blue staining assay. The data are the mean ± S.D. of three independent experiments. *p < 0.05. (C) IFNα increases the apoptosis induced by TNF-α plus CHX in HeLa cells. HeLa cells were plated in 12-well plates, and the next day the cells were untreated or treated with IFNα for 12 h and then treated with TNF-α plus CHX for the indicated times (TNF-α: 5 ng/ml, CHX: 5 μg/ml). (D) The ratio of cell death in (C) was determined by a Trypan blue staining assay. The results are representative of three independent experiments, and the error bars represent the SD. *p < 0.05, **p < 0.01.