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. 2017 Aug 17;117(8):1164–1175. doi: 10.1038/bjc.2017.260

Figure 5.

Figure 5

Inhibition of NF-κB signalling by COMMD10 is necessary for FMNL2-mediated CRC cell behaviours. (A) Effects of FMNL2 and FMNL2/COMMD10 on the levels of key proteins of NF-κB signalling pathway. (B) Effects of shFMNL2 and shFMNL2/shCOMMD10 on the levels of key proteins of NF-κB signalling pathway. (C) Effects of FMNL2, FMNL2/COMMD10, shFMNL2 and shFMNL2/shCOMMD10 on the proliferation of SW480 or SW620 cells in vitro by CCK-8 arrays. *P<0.001. (D) Effect of FMNL2, FMNL2/COMMD10, shFMNL2 and shFMNL2/shCOMMD10 on the invasion of SW480 or SW620 cells in vitro by Boyden chamber. Morphological comparison of cells penetrating the artificial basement membrane was also shown. Scale bars represented 50 μm. (E) Images of subcutaneous tumours of mice injected with SW620/NC or SW620/shFMNL2 or SW620/shFMNL2/shCOMMD10 stable transfected cells. The expressions of FMNL2, COMMD10 and p65 in the shFMNL2 tumours were measured by western blot. (F) Effects of shFMNL2 and shFMNL2/shCOMMD10 on lung metastasis in vivo. SW620/NC or SW620/shFMNL2 or SW620/shFMNL2/shCOMMD10 stable transfected cells were injected through tail vein. The number of lung metastasis nodules was counted. The tumour sections were stained with H&E. Error bars represent mean±s.d. Scale bars represented 50 μm. **P<0.01; *P<0.05. A full colour version of this figure is available at the British Journal of Cancer journal online.