Fig. 2.

Detection by SDS-PAGE/immunoblotting of apolipoproteins in material captured from plasma by dynabeads cross-linked with anti-α-syn antibody C211. a Mild elution by citrate followed by immunoblotting with anti-apoA1. b Elution by SDS followed by immunoblotting with anti-apoB. c Elution by SDS followed by immunoblotting with anti-apoE. d Elution by SDS followed by immunoblotting with anti-apoJ. In each case: Lane 1 = apolipoprotein standard; Lane 2 = eluant from beads exposed to plasma; Lane 3 = eluant from unconjugated beads exposed to plasma; Lane 4 = eluant from conjugated beads exposed to PBS. Numbers on LHS indicate positions of MW standards (in kDa). Bands corresponding to apoA1, apoB, apoE, and apoJ are also indicated