Fig. 3.

Detection of α-syn by anti-α-syn antibody C211in material captured from plasma by dynabeads cross-linked with anti-apolipoprotein antibodies. Lane 1 = 50 ng of R-α-syn as positive control; Lane 2 = whole plasma as a positive control; Lane 3 = eluant from unconjugated beads exposed to plasma as a negative control; Lane 4 = eluant from conjugated beads with anti-apoB antibody exposed to plasma. Lane 5 = eluant from conjugated beads with anti-apoA1 antibody exposed to plasma. Lane 6 = eluant from conjugated beads with anti-apoJ antibody exposed to plasma. Lane 7 = eluant from conjugated beads with anti-apoE antibody exposed to plasma. Lane 8 = eluant from conjugated beads with anti-apoB antibody exposed to PBS. Lane 9 = eluant from conjugated beads with anti-apoA1 antibody exposed to PBS. Lane 10 = eluant from conjugated beads with anti-apoJ antibody exposed to PBS. Lane 11 = eluant from conjugated beads with anti-apoE antibody. Numbers on LHS indicate positions of MW standards (in kDa). Bands corresponding to α-syn are also indicated