Fig. 5.

FABP5 deficiency enhances endothelial cell survival. a FABP5-KD and control HUVECs were cultured in 5 % FBS-containing medium for 24 h. Cells were harvested, stained with FITC-conjugated annexin V and propidium iodide, and analyzed by flow cytometry. Representative plots and mean results ± SEM from five independent experiments are shown (*p < 0.01 vs control). b FABP5-KD and control HUVECs were cultured in 10 or 5 % FBS-containing media for 24 h. Cells were harvested, and immunoblotting for caspase 3 was performed. c FABP5-KD and control HUVECs were treated with TNFα (40 ng/ml) or the vehicle control for 24 h, and immunoblotting for caspase 3 was performed. All immunoblots are representative of a minimum of three independent experiments