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Expression of Burkholderia antigens. His-tagged (6×) recombinant proteins were expressed as inclusion bodies in a pET30a(+) IPTG-inducible expression vector. Inclusion bodies were purified, denatured, and solubilized into a refolding buffer prior to dialysis against PBS. (A) Protein yields per liter bacterial culture. (B) SDS-PAGE of top seven candidates and Hcp1. Lane 1, OmpW; 2, OpcP1; 3, hemagglutinin; 4, Hcp1; 5, FlgD; 6, OpcP porin; 7, porin; 8, FlgL.
