FIG 5.

Prime and two boost vaccinations with AuNP-FlgL-LPS and AuNP-combo-LPS provide 90% and 100% protection against B. pseudomallei challenge, respectively. Survival curves for AuNP glycoconjugate groups (n = 10) after i.n. challenge with 3.4 LD₅₀ of B. pseudomallei K96243. Prior to challenge, animals received a prime and two boost vaccinations (s.c.) with the following vaccine formulations: adjuvant only (▼), LPS only (□), AuNP-BSA-LPS (·), AuNP-hemagglutinin-LPS (■), AuNP-Hcp1-LPS (▲), AuNP-FlgL-LPS (⬥), and AuNP-combo-LPS (○). Animals were monitored for 35 days postchallenge. Statistical analyses were performed by the Kaplan-Meier method, followed by log-rank test. Levels of significance compared to the adjuvant-only group: **, P < 0.005; ***, P < 0.0005.