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. 2017 Nov 6;36:155. doi: 10.1186/s13046-017-0619-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — TAGLN2 knockdown decreases invasive ability of glioma cells. a Western blot of lysates (20 μg) from U87MG and U251 cells transfected with TAGLN2 and control siRNAs incubated with TAGLN2 antibody. GAPDH was used as a loading control. b Representative images of invaded spheroids in 3D invasion assay for U87MG, U251 and GBM#P3 cells transfected with TAGLN2 and control siRNAs evaluated at 48 h and 96 h are shown. Scale bar = 200 mm. c The area covered by invading cells quantitated after 96 h. NT: no treatment; Control: non-silencing siRNA; si-TAGLN2: siRNAs targeting TAGLN2