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. 2017 Sep 6;3(4):FSO243. doi: 10.4155/fsoa-2017-0074

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© 2017 Kazunari Higa

This work is licensed under a Creative Commons Attribution 4.0 License

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Figure 2. — (A) ALP staining, (B) ARS staining, (C) oil red O staining, (D) safranin O staining and (E) immunohistochemistry of Coll II in noninduced (upper panel) and induced (lower panel) human oral mucosa middle interstitial tissue fibroblasts. Scale bars: 50 μm. Reverse transcription-PCR analysis shows (F) osteogenic (ALP, OPN), (G) adipogenic (PPAR-γ, leptin) and (H) chondrogenic (BMP-6, aggrecan)-specific gene expression. Primers used are listed in Table 3. GAPDH was used as an internal control. Left lane: noninduced human oral mucosa middle interstitial tissue fibroblasts; right lane: induced human oral mucosa middle interstitial tissue fibroblasts.

ALP: Alkaline phosphatase; ARS: Alizarin red S; Aggr: Aggrecan; Coll II: Collagen type II; GAPDH: Glyceraldehyde-3-phosphate dehydogenase; OPN: Osteopontin; PPAR-γ: Peroxisome proliferator-activated receptor-γ.