Table 3. . Primer sequences and product size of reverse transcription polymerase chain reaction.
| Primer | Sequence (5′→3′) | Product size (bp) | |
|---|---|---|---|
| ALP | Alkaline phosphatase, hydrolase enzyme | GGCGGCAGACTTTGGTTT | 552 |
| CCCGTGGCAACTCTATCTT | |||
| OPN | Osteopontin, bone sialoprotein 1 | TGGCTAAACCCTGACCCATCTC | 548 |
| TAACTGTCCTTCCCACGGCTGT | |||
| PPAR-γ2 | Peroxisome proliferator-acticated receptor-gamma2, adipocyte-specific transcription factor | GGTCAGCGGGAAGGACTTTA | 516 |
| GATCCAGTGGTTGCAGATTA | |||
| Leptin | Adipose-derived hormones | GCCAGAGTTCCTTCCCTTAA | 508 |
| CAAGCTGTGCCCATCCAAAA | |||
| Aggrecan | Chondroitin sulfate proteoglycan 1 | TCCTGGAAGCTCTTCTCAGT | 510 |
| ATGCCCAAGACTACCAGTGG | |||
| BMP-6 | Bone morphogenetic protein-6, chondrogenic marker | CTCGGGGTTCATAAGGTGAA | 451 |
| ACCGCATAACATGGGGCTTC | |||
| Nestin | Neural stem cell marker, type VI intermediate filament | GCGTTGGAACAGAGGTTGGAG | 385 |
| GCACAGGTGTCTCAAGGGTAG | |||
| NFH | Neurofilament heavy chain, neuron-specific protein | TGAACACAGACGCTATGCGCTCAG | 398 |
| CACCTTTATGTGAGTGGACACAGAG | |||
| βIII-tubulin | Tubulin β-4, neuron-specific marker | GTCTTACGAGGAGCTGCAGACGCA | 233 |
| CCACATAGCTGCAGCTTGCCATCT | |||
| ALDH3A1 | Aldehyde dehydrogenase 3 family member A1 | ACTCAGCAGGACGAGCTCTAC | 495 |
| GGGTCACAGAGGATGTAGTC | |||
| Lumican | SLRP family/Class II subfamily of proteins | CCACAACAACCTGACAGAGTGT | 488 |
| CAAGTTGATTGACCTCCAGG | |||
| Keratocan | Cornea-specific KSPG | CCTCCAAGATTACCAGCCAA | 367 |
| TTCCATCCAGACGGAGGTAG | |||
| Keratin 3 | Corneal epithelium differentiation marker and palate epithelium marker | GACAATAATCGTTCCCTGG | 434 |
| TTGCGGTAGGTGGCGATCT | |||
| Keratin12 | Corneal epithelium differetiation marker | CAACGACATGAGGGCGCA | 481 |
| AATTGACTGTGCTTGAGA | |||
| Keratin14 | Stratified squamous epithelial marker | ACTACCTGCAGCCGCCAGTT | 1417 |
| CAGTTCTTGGTGCGAAGGAC | |||
| Collagen type IV | Collagen type IV, basement membrane component | ATACCTGGAAGGGCAAGAGAA | 484 |
| TGGCACCGGCTGATTTTCTGG | |||
| Laminin | Extracellular matrix protein | TGCGAGCATGTCAGGATTTC | 961 |
| TCAGTTCCAACTCTCCCATTG | |||
| GAPDH | Glyceraidehyde-3-phosphate dehydrogenase, internal control | ACCACAGTCCATGCCATCAC | 452 |
| TCCACCACCCTGTTGCTGTA | |||
KSPG: Keratan sulfate proteoglycan; SLRP: Small leucine-rich proteoglycan.