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. 2017 Sep 4;3(4):FSO244. doi: 10.4155/fsoa-2017-0079

Figure 2. . Establishment of PD-L1 and PD-L2 analysis by chipcytometry.

Figure 2. 

(A) Flow cytometry analysis. Non-small-cell lung cancer cell lines A549 and H1975 were incubated with antihuman PD-L1 and PD-L2 (black), and corresponding isotype control (gray). Cells were fixed prior to staining. (B & C) Chipcytometry analysis. A549 cells (black), H1975 cells (black) and whole blood samples collected from a healthy donor (gray) were loaded onto Zellkraftwerk ZellSafe™ chips, fixed and stained with antihuman PD-L1 and PD-L2.