Table 1.

Primers used for PCR and DNA sequencing

Virulence factor	Primer sequence 5´-3´	Position in open reading frame	Size of product	References
Stx1	TTCGCTCTGCAATAGGTA	125-142 of A subunit	555*	Frank et al. 1998
	TTCCCCAGTTCAATGTAAGAT	659-679 of A subunit
Stx2	GTGCCTGTTACTGGTTTTTCTTC	30-53 of A subunit	118	Frank et al. 1998
	AGGGGTCGATATCTCTGTCC	128-147 of A subunit
eaeA	ATATCCGTTTTAATGGCTATCT	992-1013 of eaeA	425	Frank et al. 1998
	AATCTTCTGCGTACTGTGTTCA	1395-1416 of eaeA

^* PCR condition: 35 × (94°C for 60 s, 50°C for 60 s, 72°C for 90 s)