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. 2017 Oct 24;2017:6527306. doi: 10.1155/2017/6527306

Figure 1.

Figure 1

γ-IFN responses of patients 1 and 2 to Sp17 peptides. In (a) peripheral blood mononuclear cells (PBMCs) obtained from patients 1 and 2 at time of diagnosis after one year from start of treatment were maintained in short tern culture. A significant γ-IFN response to peptides Sp17(1) and Sp17(2) was observed in cells from both patients obtained at both time points (P < 0.05). No significant response was detected in cultures stimulated by the HIV peptide or containing medium only. (b) PBMCs from patient 1 after three rounds of peptide stimulation expanded in response to Sp17(1) and Sp17(2) peptides. (c) PBMCs from patient 1 were either enriched for CD8-positive cells using anti-CD8 antibody-coated magnetic beads or incubated with an anti-HLA-A∗0201 monoclonal antibody (BB7.2). A significant γ-IFN response was observed only in the culture containing the CD∗-positive cells in the absence of anti-MHC class I (P < 0.05). No significant responses were detected in the control cultures or the irrelevant peptides. The results are mean +/− SD and were obtained from triplicate ELISPOT cultures.