Fig. 2. Activity and inhibitor binding of BtGH84_TM-4 conjugate: (a) structure of the 4MU-GlcNAc substrate used in this study. (b) Michaelis–Menten plot for the hydrolysis of 4MU-GlcNAc by BtGH84_TM-4 (pink), in comparison to native enzyme (green), unmodified BtGH84 (orange) and the non-covalent activator 3 (purple). (c) Representative ITC data for PUGNAc binding to BtGH84_TM (left) and BtGH84_TM-4 (right).