Fig. 3. Covalent activator chemical structures and X-ray crystal structures of BtGH84_TM conjugates. (a) Chemical structures of the six additional acrylamide containing compounds used to label the BtGH84_TM mutant. (b) Stereo image of the BtGH84_TM-4 conjugate (grey) X-ray crystal structure with PUGNAc (green carbons) bound to the active site. The covalent modification is highlighted (pink carbons) and the SA Fo-Fc omit map of PUGNAc and 4 is shown as green mesh contoured at 3.5σ. (c) Stereo image of the BtGH84_TM-8 conjugate (grey) X-ray crystal structure with PUGNAc (green carbons) bound to the active site. The covalent modification is highlighted (blue carbons) and the SA Fo-Fc omit map of PUGNAc and 8 is shown as green mesh contoured at 3.5σ.
