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. 2017 Oct 24;2017:7983217. doi: 10.1155/2017/7983217

Figure 3.

Figure 3

Dual TLR2/7 ligands induce limited mast cell activation directly but suppress IgE-and-antigen-induced mast cell degranulation. IL-6 secretion and cytotoxicity of stimulated BALB/c (a) and C57BL/6 (b) BMCMCs. TLR2/7 ligand-induced degranulation in the presence or absence of DNP-HSA from αDNP-IgE-sensitized C57.1 MCs. Dashed line indicates the level of β-hexosaminidase release induced by stimulation with 10 ng/mL DNP-HSA alone (c). Analysis of phospho-ERK1/2 (Thr202/Tyr204) levels in C57BL/6 BMCMCs stimulated with 5 μM of the different TLR ligands for 5 min (d). Grey: unstained cells; grey tinted: unstimulated; black: stimulated as indicated; Data are representative results of two (d) or mean of three (a, b, c) independent experiments ± SD. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.