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. Author manuscript; available in PMC: 2018 Apr 18.
Published in final edited form as: Immunity. 2017 Apr 18;46(4):621–634. doi: 10.1016/j.immuni.2017.03.020

Figure 4. NLRP14 interacts with components of cytosolic nucleic acid sensing machinery.

Figure 4

(A) NLRP14-Flag was coexpressed with HA-cGAS or STING-HA in 293T cells and subjected to immunoprecipitation (IP) and immunoblotting (IB) using appropriate antibodies. (B) Myc-NLRP14 was coexpressed with or without HA-cGAS in 293T-STING-HA cells and subjected to IP and IB using the appropriate antibodies. (C) The effect of NLRP14 on TBK1-STING interaction was examined in the context of 1μg (+) or 2μg (++) Myc-NLRP14 expression plasmid. Myc-NLRP14 was coexpressed with (D) RIG-I-Flag, MAVS-Flag, TBK1-Flag, or IRF3-Flag (E) FL-MAVS or FL-MAVSΔTM (F) FL-STING or FL-STING(I200N) in 293T cells and monitored for interactions by IP followed by IB using appropriate antibodies. WCL, HC and LC indicate whole cell lysates, Heavy-Chain, and Light-Chain, respectively. (See also Supplemental Figure S6)